Code | Date | City | Fees | Register |
---|---|---|---|---|
T004 | June 9, 2024 - June 13, 2024 | Dubai – UAE | $ 5000 |
Register Course.. |
T004 | September 1, 2024 - September 3, 2024 | Dubai – UAE | $ 3000 |
Register Course.. |
T004 | December 8, 2024 - December 12, 2024 | Online | $ 1500 |
Register Course.. |
T004 | March 23, 2025 - March 27, 2025 | Vienna | $ 5000 |
Register Course.. |
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Objectives
- Upon Completion of the Course, Participants will be able to:
- The aim is to provide first hand information to the participants on optimal use of gas chromatograph (GC).
- A special emphasis has been given on the demonstration of applications, maintenance and troubleshooting of GC.
- Imparting participants’ fundamental knowledge of Gas Chromatography as a technique.
- To understand GC maintenance methods as a routine checks.
- Knowledge of accessories and consumables required for its operation.To familiarize participants to the techniques for achieving gas chromatography analysis, qualitative and quantitative methods, cause and effect diagrams and standard curve.
The Delegates
- All who works in any laboratory, i.e. clinical, industry, gas, petroleum, refinery, pharmacy, etc.
- Laboratory manager and supervisor
- Laboratory engineers and chemists
- Laboratory analysts and techniciansInstrument engineers/technicians
Contents
- Theory Parameters:
- Plate height(H),
- Retention Time,
- Capacity Factor,
- Selectivity Factor,
- Column Efficiency,
- Column Resolution.
- Sampling And Sample Handling Iso 17025:
- Requirements for Gases,
- Liquid and Solid,
- Sample Sampling Methods,
- Sample Registration and Storage,
- Sample Disposal,
- Max Holding Time of Sample,
- Sample Contamination.
- Sample Preparation And Analyte Manipulation Technique:
- Solid-phase extraction,
- Static head space technique,
- Soxhlet extraction,
- Microwave-assisted extraction,
- Super critical fluid extraction
- Derivatization Method:
- Silyl, – Alkyl,
- Acylation and Esterfication Methods
- Types Of GC:
- Normal GC, High Speed GC and Portable GC
- Gc Separation And Retention Process:
- Dispersive Interactions,
- Dipole-Dipole Interactions,
- Dipole-Induced Dipole Interactions
- Van Deemter equation,
- Optimal Practical Gas Velocity(OPGV),
- Average Linear Velocity,
- Flow Rate,
- Carrier Gas Selection,
- Pressure Regulator,
- Gas Purification
- Sample Injection System:
- Sample Introduction Technique,
- Manual Injection,
- Solvent Flush Injection,
- Septa,
- Inlet Liner,
- Split/Split less Inlets System,
- Spit Ratio,
- Purge Delay Time,
- Cool On-Column Inlets,
- Programmed Temperature Vaporization,
- Headspace Technique,
- Injection Problems,
- Hot Vaporizing Injector
- Column Configurations:
- Stationary Phase of Packed and Capillary Column,
- Packed Columns,
- Temperature programming,
- Gas-Liquid and Gas- Solid Columns,
- Polarity and Selectivity,
- Column Coating
- Detectors:
- Ideal Characteristics,
- Column Position,
- Gases,
- Flame Ionization Detector,
- Thermal Conductivity Detector,
- Flame Photometric Detector,
- Nitrogen Phosphorous Detector,
- Photo Ionization Detector,
- Electron Capture Detector,
- Mass Spectrometry(Fragmentation and identify technique with software training),
- Chemiluminescent Detector,
- Mass Analyzer – Time of Flight,
- Ion Trap and Quadrupole Technique.
- Maintenance And Installation Methods:
- Clean and Condition Septa,
- Cleaning Injector Liners,
- Silylating Liners,
- Column Conditioning,
- Installation Fused Silica Capillary Columns,
- Column Placement in the oven,
- Column Installation,
- Leak Detection,
- Bleed Test,
- FidJet Cleaning Procedure,
- TCD Clean Detector Cell,
- Cleaning Of ECD,
- FPD Maintenance,
- Cleaning MS and Change The Filament
- Instrument Problems And Troubleshooting:
- Approaches to Solve GC Problems,
- Troubleshooting Tests,
- Band Broadening,
- Broadening Initial Peak Band widths,
- Retention Gap Sampling,
- Sampling By Solute Focusing,
- Retention Gaps Tube,
- Baseline Deviation,
- Noisy Baseline,
- Spikes in Baseline,
- Peak Shape Problems,
- Flat Top Peaks,
- Split Peaks,
- Negative Peaks,
- Retention Changes,
- Ghost Peak,
- Causes and Prevention of Column Damage,
- Column Contamination,
- Needle Discrimination,
- Change in Detectors Sensitivity,
- Difficulty in Lighting Fid Flame,
- Loss of Detectors Linear Range,
- Leaks in MS,
- Excessive Noise or High Background in MS
- Practical Demonstration:
- Introduction and familiarization with GC,
- Preparation of sample test,
- Preparation of standards,
- Setting the methods program,
- Inject the sample and standards,
- Print the chromatograms,
- Check all GC components and how to do the preventive maintenance
- Quality Control And Validation Method:
- Specificity,
- Linearity,
- Selectivity,
- Accuracy,
- Precision,
- Bias,
- Linearity,
- Range,
- Limit of Detection,
- Limit Of Quantification,
- Robustness,
- Ruggedness,
- Stability
- Quantitative Methods:
- Interpolated Graph Calibration by Using External and Internal Standards,
- Standard Addition Method,
- Calibration Methods,
- Correlation Coefficient,
- Outliers Test of Repeated Measurements,
- Outliers In Calibration Curve,
- Errors In Quantitative Analysis,
- Distribution of Errors,
- Confidence Limit,
- Limit of Detection,
- Repeatability,
- Reproducibility,
- Optimization Methods
The Discount
10% in case of Three P. (or more)